SrasV1, Main, Exploration, bibRecord, 005D49

The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV

Identifieur interne : 005D49 ( Main/Exploration ); précédent : 005D48; suivant : 005D50

The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV

Source :

Genomics, Proteomics & Bioinformatics [ 1672-0229 ] ; 2003.

RBID : PMC:5172421

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Motifs (genetics), Amino Acid Sequence, Antigens, Viral (immunology), Base Composition, Base Sequence, Cluster Analysis, Computational Biology, DNA Primers, Enzyme-Linked Immunosorbent Assay, Genetic Variation, Molecular Sequence Data, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Nucleocapsid Proteins (metabolism), Phosphorylation, SARS Virus (genetics), Sequence Analysis, DNA.
MESH :
- chemical , genetics : Nucleocapsid Proteins.
- chemical , immunology : Antigens, Viral, Nucleocapsid Proteins.
- genetics : Amino Acid Motifs, SARS Virus.
- chemical , metabolism : Nucleocapsid Proteins.
- Amino Acid Sequence, Base Composition, Base Sequence, Cluster Analysis, Computational Biology, DNA Primers, Enzyme-Linked Immunosorbent Assay, Genetic Variation, Molecular Sequence Data, Phosphorylation, Sequence Analysis, DNA.

Abstract

The Coronaviridae family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The predicted high extent of phosphorylation of the N protein on multiple candidate phosphorylation sites demonstrates that it would be related to important functions, such as RNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with other viral structural proteins, the low variation rate of the N protein with regards to its size suggests its importance to the survival of the virus.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172421

DOI: 10.1016/S1672-0229(03)01018-0
PubMed: 15626344
PubMed Central: 5172421

Affiliations:

Le document en format XML

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</placeName>
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<author><name sortKey="Tian, Xiangjun" sort="Tian, Xiangjun" uniqKey="Tian X" first="Xiangjun" last="Tian">Xiangjun Tian</name>
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<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
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<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
<placeName><settlement type="city">Hangzhou</settlement>
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</placeName>
</affiliation>
</author>
<author><name sortKey="Tan, Xuehai" sort="Tan, Xuehai" uniqKey="Tan X" first="Xuehai" last="Tan">Xuehai Tan</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
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</author>
<author><name sortKey="Xu, Ningzhi" sort="Xu, Ningzhi" uniqKey="Xu N" first="Ningzhi" last="Xu">Ningzhi Xu</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zeng, Changqing" sort="Zeng, Changqing" uniqKey="Zeng C" first="Changqing" last="Zeng">Changqing Zeng</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
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</author>
<author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
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<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
<placeName><settlement type="city">Hangzhou</settlement>
<region type="province">Zhejiang</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bi, Shengli" sort="Bi, Shengli" uniqKey="Bi S" first="Shengli" last="Bi">Shengli Bi</name>
<affiliation wicri:level="1"><nlm:aff id="aff0020">Center of Disease Control and Prevention, Beijing 100050, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Center of Disease Control and Prevention, Beijing 100050</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0010">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University and Hangzhou Genomics Institute, Hangzhou 310008</wicri:regionArea>
<placeName><settlement type="city">Hangzhou</settlement>
<region type="province">Zhejiang</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Genomics, Proteomics & Bioinformatics</title>
<idno type="ISSN">1672-0229</idno>
<idno type="eISSN">2210-3244</idno>
<imprint><date when="2003">2003</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs (genetics)</term>
<term>Amino Acid Sequence</term>
<term>Antigens, Viral (immunology)</term>
<term>Base Composition</term>
<term>Base Sequence</term>
<term>Cluster Analysis</term>
<term>Computational Biology</term>
<term>DNA Primers</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Genetic Variation</term>
<term>Molecular Sequence Data</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>Phosphorylation</term>
<term>SARS Virus (genetics)</term>
<term>Sequence Analysis, DNA</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN</term>
<term>Analyse de regroupements</term>
<term>Analyse de séquence d'ADN</term>
<term>Antigènes viraux (immunologie)</term>
<term>Biologie informatique</term>
<term>Composition en bases nucléiques</term>
<term>Données de séquences moléculaires</term>
<term>Motifs d'acides aminés (génétique)</term>
<term>Phosphorylation</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (immunologie)</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Test ELISA</term>
<term>Variation génétique</term>
<term>Virus du SRAS (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term>
<term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Amino Acid Motifs</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Motifs d'acides aminés</term>
<term>Protéines nucléocapside</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term>
<term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Composition</term>
<term>Base Sequence</term>
<term>Cluster Analysis</term>
<term>Computational Biology</term>
<term>DNA Primers</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Genetic Variation</term>
<term>Molecular Sequence Data</term>
<term>Phosphorylation</term>
<term>Sequence Analysis, DNA</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term>
<term>Analyse de regroupements</term>
<term>Analyse de séquence d'ADN</term>
<term>Biologie informatique</term>
<term>Composition en bases nucléiques</term>
<term>Données de séquences moléculaires</term>
<term>Phosphorylation</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Test ELISA</term>
<term>Variation génétique</term>
</keywords>
</textClass>
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</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The <italic>Coronaviridae</italic>
 family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The predicted high extent of phosphorylation of the N protein on multiple candidate phosphorylation sites demonstrates that it would be related to important functions, such as RNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with other viral structural proteins, the low variation rate of the N protein with regards to its size suggests its importance to the survival of the virus.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Ksiazek, T G" uniqKey="Ksiazek T">T.G. Ksiazek</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hiscox, J A" uniqKey="Hiscox J">J.A. Hiscox</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wootton, S K" uniqKey="Wootton S">S.K. Wootton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Laity, J H" uniqKey="Laity J">J.H. Laity</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Molenkamp, R" uniqKey="Molenkamp R">R. Molenkamp</name>
</author>
<author><name sortKey="Spaan, W J M" uniqKey="Spaan W">W.J.M. Spaan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ding, P" uniqKey="Ding P">P. Ding</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Narayahan, K" uniqKey="Narayahan K">K. Narayahan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Caceres, J F" uniqKey="Caceres J">J.F. Cáceres</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Furuyama, S" uniqKey="Furuyama S">S. Furuyama</name>
</author>
<author><name sortKey="Bruzik, J P" uniqKey="Bruzik J">J.P. Bruzik</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wurm, T" uniqKey="Wurm T">T. Wurm</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kolaskar, A S" uniqKey="Kolaskar A">A.S. Kolaskar</name>
</author>
<author><name sortKey="Tongaonkar, P C" uniqKey="Tongaonkar P">P.C. Tongaonkar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Daginakatte, C G" uniqKey="Daginakatte C">C.G. Daginakatte</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhu, Q Y" uniqKey="Zhu Q">Q.Y. Zhu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Qin, E D" uniqKey="Qin E">E.D. Qin</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Pékin</li>
<li>Zhejiang</li>
</region>
<settlement><li>Hangzhou</li>
<li>Pékin</li>
</settlement>
<orgName><li>Université de Pékin</li>
</orgName>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Jingqiang" sort="Wang, Jingqiang" uniqKey="Wang J" first="Jingqiang" last="Wang">Jingqiang Wang</name>
</noRegion>
<name sortKey="Bi, Shengli" sort="Bi, Shengli" uniqKey="Bi S" first="Shengli" last="Bi">Shengli Bi</name>
<name sortKey="Hu, Jianfei" sort="Hu, Jianfei" uniqKey="Hu J" first="Jianfei" last="Hu">Jianfei Hu</name>
<name sortKey="Hu, Jianfei" sort="Hu, Jianfei" uniqKey="Hu J" first="Jianfei" last="Hu">Jianfei Hu</name>
<name sortKey="Hu, Yongwu" sort="Hu, Yongwu" uniqKey="Hu Y" first="Yongwu" last="Hu">Yongwu Hu</name>
<name sortKey="Ji, Jia" sort="Ji, Jia" uniqKey="Ji J" first="Jia" last="Ji">Jia Ji</name>
<name sortKey="Li, Dawei" sort="Li, Dawei" uniqKey="Li D" first="Dawei" last="Li">Dawei Li</name>
<name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
<name sortKey="Sun, Min" sort="Sun, Min" uniqKey="Sun M" first="Min" last="Sun">Min Sun</name>
<name sortKey="Tan, Xuehai" sort="Tan, Xuehai" uniqKey="Tan X" first="Xuehai" last="Tan">Xuehai Tan</name>
<name sortKey="Tian, Xiangjun" sort="Tian, Xiangjun" uniqKey="Tian X" first="Xiangjun" last="Tian">Xiangjun Tian</name>
<name sortKey="Tian, Xiangjun" sort="Tian, Xiangjun" uniqKey="Tian X" first="Xiangjun" last="Tian">Xiangjun Tian</name>
<name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name>
<name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name>
<name sortKey="Wang, Jingqiang" sort="Wang, Jingqiang" uniqKey="Wang J" first="Jingqiang" last="Wang">Jingqiang Wang</name>
<name sortKey="Wen, Jie" sort="Wen, Jie" uniqKey="Wen J" first="Jie" last="Wen">Jie Wen</name>
<name sortKey="Xu, Ningzhi" sort="Xu, Ningzhi" uniqKey="Xu N" first="Ningzhi" last="Xu">Ningzhi Xu</name>
<name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name>
<name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name>
<name sortKey="Ye, Jia" sort="Ye, Jia" uniqKey="Ye J" first="Jia" last="Ye">Jia Ye</name>
<name sortKey="Ye, Jia" sort="Ye, Jia" uniqKey="Ye J" first="Jia" last="Ye">Jia Ye</name>
<name sortKey="Zeng, Changqing" sort="Zeng, Changqing" uniqKey="Zeng C" first="Changqing" last="Zeng">Changqing Zeng</name>
<name sortKey="Zeng, Haipan" sort="Zeng, Haipan" uniqKey="Zeng H" first="Haipan" last="Zeng">Haipan Zeng</name>
<name sortKey="Zhao, Xiaoqian" sort="Zhao, Xiaoqian" uniqKey="Zhao X" first="Xiaoqian" last="Zhao">Xiaoqian Zhao</name>
</country>
</tree>
</affiliations>
</record>

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The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV

The Structure Analysis and Antigenicity Study of the N Protein of SARS-CoV

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